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By Rodolfo Paoletti, Dr. David Kritchevsky

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And C« = Ci_i + dH i = 1, 3, 5, The expanded integral curve consists of these d paired with F* and X*. Using the tables of C and X values, m% can be calculated for each standard interval by linear interpolation: the desired value will corre­ spond to the concentration (Ci + Ci_i)/2. This permits the standard cor­ rection to base of cell for each component (15) according to: dBC = d (mi/xo)2 Before output, the CiBG are divided by an appropriate factor that relates them to the original lipoprotein sample.

0630 gm/ml). III. A Generalized Method for the Computer Analysis of Lipoprotein Distributions Although it is possible to read schlieren films with automatic scanning devices (13) that produce data in a form suitable for direct use on a computer, we have elected to use a simple manual procedure in prepar­ ing analytical ultracentrifuge data for computer analysis. This not only reduces cost in the developmental phase of the computer solution, but also allows greater control over such reading problems as accurate sens- Human Serum Lipoprotein Distributions 33 ing of base of cell on the film, and correct separation of the intersecting schlieren patterns that may result from simultaneous runs on the ultracentrifuge.

35 mm with respect to the usual centerpiece. In loading a double-sectored cell, great care should be exercised in order to fill each sector with as nearly the same solution volume as is pos­ sible. This is required for baseline accuracy as well as to minimize diffi­ culties that would occur during the run if fluid exchanged between the two sectors without actual cell leakage. 42-ml delivery for both the high-density and low-density runs. Because of the substantial time, expense, and technical effort required to make an ultracentrifugal analysis, it is recommended that the lowdensity lipoprotein fraction first be analyzed by refractometry (8) to determine accurately the total low-density serum lipoprotein concentra­ tion in each sample.

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